Deuterated hen egg-white lysozyme crystals: Optimization of the growth conditions and morphology

Autor:	L. A. Shuvalov, A. V. Svanidze, Sergey Lushnikov
Rok vydání:	2005
Předmět:	Morphology (linguistics) Sodium chemistry.chemical_element Protonation General Chemistry Condensed Matter Physics law.invention chemistry.chemical_compound Tetragonal crystal system Crystallography chemistry Deuterium law General Materials Science Lysozyme Crystallization
Zdroj:	Crystallography Reports. 50:789-795
ISSN:	1562-689X 1063-7745
DOI:	10.1134/1.2049397
Popis:	Deuterated and protonated tetragonal lysozyme crystals are grown using the hanging-drop vapor-diffusion method. The size of the lysozyme crystals grown is determined as a function of the concentration of sodium chloride used as a precipitant. It is found that crystallization leads to the formation of lysozyme crystals with three different habits. Morphological and X-ray diffraction analyses of the deuterated and protonated lysozyme crystals demonstrate that, despite the different habits, all the crystals grown belong to the tetragonal crystal system. The simple forms of lysozyme crystals are revealed. It is shown that the habits of the lysozyme crystals are determined by the specific combinations of simple forms. The mechanisms responsible for the formation of lysozyme crystals with different habits are discussed.
Databáze:	OpenAIRE
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