The study of single cells in a system based on Silicon Photomultipliers
| Autor: | Wojciech Kucewicz, Mateusz Baszczyk, Piotr Dorosz |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Materials science
Laser diode business.industry 010401 analytical chemistry 02 engineering and technology Integrated circuit 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence 0104 chemical sciences law.invention Software Silicon photomultiplier law Calibration Optoelectronics Electronics 0210 nano-technology Optical filter business |
| Zdroj: | 2018 IEEE Nuclear Science Symposium and Medical Imaging Conference Proceedings (NSS/MIC). |
| DOI: | 10.1109/nssmic.2018.8824598 |
| Popis: | This paper presents an application for single molecule detection based on the silicon photomultiplier (SiPM). The research methodology uses the principles of fluorescence measurement. Single molecule detection enables reducing the required volume of the measured sample by a considerable degree. This allows the study of complex reaction mechanisms, rare events, or subpopulations of complex biomolecules such as DNA or proteins whose individual properties are often hidden in conventional ensemble-based measurements. This paper describes a complete readout system designed for fluorescence detection. The system consists of a SiPM with a dedicated application-specific integrated circuit, and a software application for saving and evaluating data. Aside from the electronics, the readout is equipped with an optical system (a laser diode, a band-pass optical filter and a µ-Slide plate). The system has been applied to the fluorescence detection of fluorophores. The obtained results provide the basis for performing fluorescence measurements of single cells in a multi-spot optical system. The calibration of the system has been performed with the use of fluorescent cytometer dye. |
| Databáze: | OpenAIRE |
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