Expression and Preparation of Recombinant Reelin and ADAMTS-3 Proteins
| Autor: | Takao Kohno, Yuko Yamakage, Himari Ogino, Mitsuharu Hattori |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Thrombospondin Proteases Metalloproteinase biology Chemistry ADAMTS Cleavage (embryo) law.invention Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine nervous system law Recombinant DNA Disintegrin biology.protein Reelin 030217 neurology & neurosurgery |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493996971 |
| DOI: | 10.1007/978-1-4939-9698-8_8 |
| Popis: | Reelin is a large secreted protein that is essential for the brain development and function. Reelin is negatively regulated by the specific cleavage by a disintegrin and metalloproteinase with thrombospondin type 1 motifs 3 (ADAMTS-3) which is also secreted from neurons. It is likely that there are other proteases that can cleave Reelin. This chapter describes the protocol for expression and handling of recombinant Reelin and ADAMTS-3 proteins to facilitate investigation of these proteins. |
| Databáze: | OpenAIRE |
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