Investigation on the photo-induced de-oxygenation process of myoglobin in aqueous solution by use of fluorescence spectroscopy

Autor:	JiHan Hu, Qian Tang, Da-bin Gao, Xue-Fang Zheng, Jing Ma, Jun-yan Ma
Rok vydání:	2008
Předmět:	Aqueous solution Chemistry Tryptophan Analytical chemistry chemistry.chemical_element General Chemistry Photochemistry Oxygen Fluorescence spectroscopy chemistry.chemical_compound Myoglobin Molecule Fluorescence cross-correlation spectroscopy Laser-induced fluorescence
Zdroj:	Science in China Series B: Chemistry. 51:414-419
ISSN:	1862-2771 1006-9291
DOI:	10.1007/s11426-008-0014-7
Popis:	A photo-induced de-oxygenation process of myoglobin (Mb) in aqueous solution was investigated by use of fluorescence spectroscopy. The spectra are characterized by the fluorescence intensity declining gradually after each scan, and the decay of fluorescence intensity being significant in each scan, which is assigned to the release of oxygen from the opening of the heme-pockets induced by illumination. More illumination will cause more release of oxygen; if the temperature of an Mb solution is increased when it is illuminated, the rate of de-oxygenation will be higher. It was found that ligand-oxygen in Fe-porphyrin could be removed from Mb by nitrogen. This indicates that the interaction between oxy-Mb and other different gases can be tested by the method of fluorescence spectroscopy. In addition, fluorescence spectroscopy can be employed to probe the energy transfer between Fe-porphyrin and tryptophan or tyrosine in Mb molecules.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::86481f32212f47afd28a5a94e6f72d36 https://doi.org/10.1007/s11426-008-0014-7 Zobrazit plný text záznamu Full text from SpringerLink