Fluorescent brightener inhibits apoptosis in baculovirus-infected gypsy moth larval midgut cellsin vitro
| Autor: | Neelam Narang, Martin Shapiro, Marcia J. Loeb, Edward M. Dougherty, Dwight E. Lynn |
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| Rok vydání: | 2006 |
| Předmět: | |
| Zdroj: | Biocontrol Science and Technology. 16:157-168 |
| ISSN: | 1360-0478 0958-3157 |
| Popis: | Fluorescent brighteners significantly lower the LC50 and LT50 in a variety of nucleopolyhedrovirus–insect host systems. In larvae of the gypsy moth, Lymantria dispar (L.), a European NPV strain of virus (LdMNPV) does not normally replicate in the midgut, but addition of a fluorescent brightener (Calcofluor M2R) to the virus suspension results in productive infections. In the current study, we show that LdMNPV also does not replicate in a larval midgut primary cell culture system unless a fluorescent brightener (Blankophor P167) is added. Morphological and cellular changes characteristic of apoptotic cell death were noted in infected midgut cells in vitro. We used the TUNEL assay to measure apoptosis in virus-challenged midgut cell cultures at 24–48 h post-inoculation. A significant decrease in apoptotic midgut cells was noted in the presence of 0.01 M brightener. The inhibition of apoptosis and presumptive inhibition of shedding of infected midgut cells in the presence of fluorescent brightener i... |
| Databáze: | OpenAIRE |
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