Comparison of Growth of Trichomonas foetus and Trichomonas vaginalis in Chick Embryos
| Autor: | Ray E. Trussell, S. H. McNutt |
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| Rok vydání: | 1941 |
| Předmět: | |
| Zdroj: | Experimental Biology and Medicine. 46:489-492 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-46-12038 |
| Popis: | Nelson1 first reported the cultivation of Trichomonas foetus in chick embryos after inoculations of 1 cc of a culture into the allantoic sac. The eggs were opened 3 to 4 days after inoculation. Allantoic fluid from the most heavily infected embryos was used to carry the protozoan through 14 subcultures. Later, Levine, Brandly, and Graham,2 following the same procedure, carried T. foetus through 23 passages and found that 12-day embryos were better than others in an 8- to 16-day series tested. Hogue3 inoculated T. foetus into the allantoic sac of developing chick embryos and allowed the embryos to hatch or die. In one of 4 chicks that hatched, the protozoa were found in the yolk sac, esophagus, intestines, gall bladder, and cloaca 2 days after hatching. The other 3 gave negative results on fecal examinations. In embryos that died after inoculation, the trichomonads were also found in the digestive tract, yolk, and gall bladder.The present report concerns a comparison of the growth of bacteria-free cultures... |
| Databáze: | OpenAIRE |
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