A 45 kDa protein isolated from the nodal walls ofChara corallinais localised to plasmodesmata

Autor: Brian E. S. Gunning, Leila M. Blackman, Robyn L. Overall
Rok vydání: 1998
Předmět:
Zdroj: The Plant Journal. 15:401-411
ISSN: 1365-313X
0960-7412
Popis: Summary The cellular anatomy of the green alga,Chara corallina,was exploited to isolate putative plasmodesmataassociated proteins. InC. corallina, large internodal cells are symplastically connected via intervening nodal complexes of smaller cells which have plasmodesmata in their cell walls. Comparison of proteins extracted from walls with plasmodesmata (nodal complexes) with those from walls without plasmodesmata (external internodal walls) identified four putative plasmodesmata-associated proteins. These putative plasmodesmata-associated proteins were approximately 95, 45, 44 and 33 kDa. A monoclonal antibody (MAB45/22) was raised against the 45 kDa putative plasmodesmata-associated protein (CPAP45). Using immunofluorescence, this antibody co-localised with aniline blue induced fluorescence of callose in the source cell walls. MAB45/22 was localised to the plasmodesmata ofC. corallinaand, in particular, to the central cavity using immunogold cytochemistry. In contrast, a monoclonal antibody to callose specifically labelled the mouth ofC. corallinaplasmodesmata. MAB45/22 also labelled higher plant plasmodesmata.
Databáze: OpenAIRE
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