Autor:	Patrick Couvreur, Le Doan T, Francis Puisieux, Saison-Behmoaras T, C Helene, Chavany C
Rok vydání:	1994
Předmět:	Pharmacology Nuclease biology Chemistry Oligonucleotide Organic Chemistry Endocytic cycle Pharmaceutical Science Nanoparticle In vitro Adsorption Biochemistry Cell culture Biophysics biology.protein Molecular Medicine Pharmacology (medical) Intracellular Biotechnology
Zdroj:	Pharmaceutical Research. 11:1370-1378
ISSN:	0724-8741
DOI:	10.1023/a:1018923301967
Popis:	Oligonucleotides can be adsorbed on polyisohexylcyanoacrylate nanoparticles in the presence of hydrophobic quartenary ammonium salts. Oligonucleotides bound to nanoparticles are protected from nuclease attack both in buffer and in cell culture media. Cellular uptake of Oligonucleotides is increased when they are adsorbed onto nanoparticles as a result of the capture of nanoparticles by an endocytic/phagocytic pathway. Intracellular stability towards nucleolytic degradation is increased in the presence of nanoparticles. These results show that nanoparticles can be considered as convenient carriers for the protection and delivery of oligonucleotides to cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8629eade139bd104af0e5ef461f9ce5c https://doi.org/10.1023/a:1018923301967 Zobrazit plný text záznamu Full text from SpringerLink