Transcriptional regulation of CacyBP/SIP gene and the influence of increased CacyBP/SIP level on gene expression pattern in colorectal cancer HCT116 cells
| Autor: | Wiesława Leśniak, Paweł Bieganowski, Anna Filipek, Beata Kądziołka, Konrad J. Dębski |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Activator (genetics) Microarray analysis techniques Clinical Biochemistry Promoter Cell Biology Biology CREB Biochemistry Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis Gene expression Genetics biology.protein Transcriptional regulation E2F1 Molecular Biology Transcription factor |
| Zdroj: | IUBMB Life. 70:50-59 |
| ISSN: | 1521-6543 |
| DOI: | 10.1002/iub.1698 |
| Popis: | The CacyBP/SIP protein is expressed at a particularly high level in brain, spleen, and various tumors. In this work, we have studied transcriptional regulation of the CacyBP/SIP gene and the influence of increased CacyBP/SIP level on gene expression in colorectal cancer HCT116 cells. We have shown that E2F1, EGR1, and CREB transcription factors bind to the CacyBP/SIP gene promoter and stimulate transcription of CacyBP/SIP gene. The role of CREB was further confirmed by the observation that forskolin, a strong activator of CREB phosphorylation/activity, increased CacyBP/SIP gene promoter activity. Moreover, we have shown that CREB dominant negative mutants, CREB133 and KCREB, inhibits CacyBP/SIP promoter activity. To check the biological significance of increased CacyBP/SIP expression/level we have applied RNA microarray analysis and have found that upregulation of CacyBP/SIP entails changes in mRNA level of many genes involved, among others, in immune processes. © 2017 IUBMB Life, 70(1):50-59, 2018. |
| Databáze: | OpenAIRE |
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