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This chapter explores the effects of alternative buffer formulations on resolution of separated cells in the McDonnell-Douglas continuous flow electrophoresis system. Specifically, organic zwitterionic buffers were selected for analysis of their utility for viable cell separations by preparative zonal electrophoresis. The availability of zwitterionic buffers formulated specifically for cell electrophoresis then prompted a comparison of these buffers with standard buffers used in past cell separation studies. Q buffer was very stable throughout the width of the electrophoresis buffer column, with only five tubes on the cathode side showing conductivity differences, these were very minor. The subsequent studies with the standard triethanolamine buffers suggested that they were unstable in the electrical field and appeared to be chemically breaking down or were either gaining or losing ions to the anode or cathode chambers. Studies of a zwitterionic buffer formulated for cell electrophoresis were done using the McDonnell-Douglas continuous flow electrophoresis system. |
