| Autor: |
Craig Koina, David M. Ashley, Andrew W. Roberts, Fiona J. Radcliff, Susan L. Woulfe, Marcia J. Riordan, Charles Baum, Denise A. Caruso, Mimi L.K. Tang |
| Rok vydání: |
2002 |
| Předmět: |
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| Zdroj: |
British Journal of Haematology. 119:204-211 |
| ISSN: |
0007-1048 |
| DOI: |
10.1046/j.1365-2141.2002.03717.x |
| Popis: |
Summary. The number of dendritic cells (DC) circulating in the peripheral blood of cancer patients were monitored at multiple time points during chemotherapy and granulocyte colony-stimulating factor (G-CSF) support. DC were identified via the lack of expression of standard lineage markers and high expression of HLA-DR (LN–/DR+). The expression of DC-associated markers, including CD83, CD11c, IL-3Rα (CDw123) and CD86, within this LN–/DR+ population was also monitored. Maximal mobilization occurred during recovery on d 12, with a mean 32-fold increase in LN–/DR+ numbers. The most striking increase was observed in the LN–/DR+/CD83+ cell population: 12 d after commencement of treatment, the proportion of these cells had increased by approximately 120-fold when compared with baseline. Peripheral blood mononuclear cell (PBMC) and CD34+ cell numbers also peaked 12 d into the treatment regimen in most patients. These data suggest that it should be possible to acquire substantial numbers of DC from leukapheresis products collected from cancer patients undergoing a standard treatment regimen of chemotherapy and G-CSF. This strategy may be a feasible, low-risk means of acquiring cells for DC-based vaccine studies. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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