Molecular cytogenetic study in Octopus (Amphioctopus) areolatus from Japan

Autor:	Tatsuki Yoshinaga, Sei-ichi Okumura, Kenta Adachi, Takashi Kuramochi, Keiko Ohnishi
Rok vydání:	2014
Předmět:	Genetics medicine.diagnostic_test Karyotype Aquatic Science Biology biology.organism_classification Genome Gene mapping Gene duplication Octopus (genus) C-value medicine Genome size Fluorescence in situ hybridization
Zdroj:	Fisheries Science. 80:445-450
ISSN:	1444-2906 0919-9268
Popis:	Octopus (Amphioctopus) areolatus is an important marine cephalopod in Japan. We examined its diploid chromosome number, karyotype, and genome size (C value), and performed fluorescence in situ hybridization (FISH) using a vertebrate telomeric probe. The diploid chromosome number was 60 in embryonic cells, with 24 pairs of metacentric chromosomes, four pairs of metacentric/submetacentric chromosomes, and two pairs of submetacentric chromosomes. The mean C value was estimated to be 5.47 pg. From these findings, and those reported previously for other octopus species, we suggest that the factor causing the quantum change of C value in O. (A.) areolatus was genome duplication, and not polyploidy. In telomeric FISH analysis, hybridization signals were clearly observed in the telomeric regions of the chromosomes. This is the first report of FISH analysis of cephalopod chromosomes, and our findings suggest that the telomere sequence of O. (A.) areolatus is (TTAGGG) n , which may allow gene mapping in the future.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::86121cc726e1d109b95ea7709a6cf772 https://doi.org/10.1007/s12562-014-0703-4 Zobrazit plný text záznamu Plný text ve formátu PDF