Trans-esterification of fatty acids from microorganisms and human blood serum by trimethylsulfonium hydroxide (TMSH) for GC analysis
| Autor: | H. P. Nalik, Gerhard Schomburg, K.-D. Müller, H. Husmann |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Chemistry Biomolecule Glyceride Organic Chemistry Clinical Biochemistry Fatty acid Transesterification biology.organism_classification Biochemistry Analytical Chemistry Blood serum Organic chemistry Sample preparation Gas chromatography Bacteria |
| Zdroj: | Chromatographia. 30:245-248 |
| ISSN: | 1612-1112 0009-5893 |
| DOI: | 10.1007/bf02319701 |
| Popis: | Trimethylsulfonium hydroxide (TMSH) can convert fatty acids into the corresponding fatty acid methyl esters (FAMEs) in a single step. These fatty acids may also be bound in biomolecules such as phospholipids and/or glycerides. Complex mixtures of saturated and unsaturated FAMEs which may contain hydroxy and cylopropyl groups are obtained by trans-esterification; they can easily be separated in most cases by capillary GC. When FAMEs are generated from different microorganisms e.g. bacteria the patterns of the chromatograms are characteristic. Examples of characteristic patterns of bacteria with different cell wall structures are shown. The described method of transesterification can also be applied directly to blood serum without sophisticated sample pretreatment. The profiles of the chromatograms match well those described in the literature obtained by other methods of trans-esterification or sample preparation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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