Effect of glucose as a carbon repressor on the extracellular proteome of Aspergillus niger during the production of amylases by solid state cultivation
| Autor: | Gabriela Carrillo-Sancen, Araceli Tomasini-Campocosio, Ulises Carrasco-Navarro, Gerardo Corzo, Martha Pedraza-Escalona, Ernesto Favela-Torres |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Signal peptide chemistry.chemical_classification biology Starch 030106 microbiology Aspergillus niger Catabolite repression Repressor Bioengineering biology.organism_classification Applied Microbiology and Biotechnology Biochemistry 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Enzyme chemistry biology.protein Extracellular Amylase |
| Zdroj: | Process Biochemistry. 51:2001-2010 |
| ISSN: | 1359-5113 |
| DOI: | 10.1016/j.procbio.2016.09.001 |
| Popis: | In comparison to traditional submerged cultivations, solid state cultivation (SSC) has been shown to reduce the effect of carbon catabolite repression during the production of important industrial enzymes, such as amylases. Nevertheless, the effect of a carbon repressor on the entire secretome during the production of enzymes of industrial interest has not been described. Therefore, the secretome composition and amylase production from Aspergillus niger grown in SSC using media containing starch and different concentrations of glucose (60 and 120 g/L) were evaluated. The results showed that proteins without an amino-terminal signal peptide, which is necessary for secretion through the classical secretory pathway, were detected at high glucose concentrations; some of these proteins were moonlighting proteins associated with pathogenesis. High concentrations of glucose also produced better amylase volumetric activities. α-Amylase activity was eight times higher in media containing glucose as compared to the medium containing only starch. Glucoamylase activity was 29 and 20 times higher at glucose concentrations of 60 and 120 g/L, respectively, compared to the medium containing only starch. Moreover, the presence of glucose had a variable effect on the abundance of the amylase isoforms produced by A. niger. |
| Databáze: | OpenAIRE |
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