[Homoserine31]-Salmon Calcitonin I: Fully Active Analogue of Calcitonin Synthesized by Recombinant DNA Techniques
Autor: | Hunter Heath, David N Fass, Flora Chow, Tomas Kempe |
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Rok vydání: | 1988 |
Předmět: |
endocrine system
animal diseases Biomedical Engineering Homoserine lac operon Bioengineering Biological activity Biology medicine.disease_cause Applied Microbiology and Biotechnology Fusion protein Molecular biology law.invention chemistry.chemical_compound chemistry Biochemistry Calcitonin law medicine Recombinant DNA Molecular Medicine Cyanogen bromide Escherichia coli hormones hormone substitutes and hormone antagonists Biotechnology |
Zdroj: | Nature Biotechnology. 6:190-192 |
ISSN: | 1546-1696 1087-0156 |
DOI: | 10.1038/nbt0288-190 |
Popis: | We have made an active analogue of salmon calcitonin I, [homoserine 31]–salmon calcitonin I, by recombinant DNA and chemical techniques. First a calcitonin precursor, the [homoserine31]–salmon calcitonin I–(1–31) lactone, was obtained from a multi–copy fusion protein upon cyanogen bromide cleavage. The protein was expressed from a multiple–copy salmon calcitonin I gene fused to lacZ. The C–terminal homoserine was reacted with prolinamide to get the 32 amino acid peptide, [homoserine31]–salmon calcitonin I. This analogue was equipotent to the naturally–occurring salmon calcitonin I in lowering plasma calcium levels in a rat bioassay. This method thus offers an opportunity to make C–terminal amides through intermediates made by recombinant DNA methods. |
Databáze: | OpenAIRE |
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