Adsorption of fibrinogen derivatives on insolubilized fibrinogen and fibrinmonomer

Autor:	D.L. Heene, F.R. Matthias
Rok vydání:	1973
Předmět:	Ethanol Chromatography Elution Complex formation Hematology Fibrinogen chemistry.chemical_compound Adsorption Thrombin chemistry medicine Agarose Fibrinmonomer medicine.drug
Zdroj:	Thrombosis Research. 2:137-154
ISSN:	0049-3848
DOI:	10.1016/0049-3848(73)90025-x
Popis:	Insolubilized fibrinogen was prepared by chemical fixation of purified fibrinogen to CNBr activated agarose. Under optimal preparative conditions a fibrinogen/agarose ratio of 1:1 was achieved on the dry weight basis. Chromatographic experiments using FG-ag columns on thrombin incubated plasma containing ethanol test positive material (fibrinmonomer) revealed adsorption of a fibrinogen derivative eluted at pH 4.1 the amount of which correlated significantly to the quantitative ethanol test most likely indicating adsorption of fibrinmonomer. Fibrinogen was considerably adsorbed on FM-ag columns under the same conditions. The results are in favour of the interaction of fibrinogen and fibrinmonomer and possible complex formation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::85e3989f1803ccc62f2ea1cc4609e6c1 https://doi.org/10.1016/0049-3848(73)90025-x Zobrazit plný text záznamu