Effects of Bleomycin on Mouse Bone-Marrow Stem Cells2
| Autor: | F Mauro, L Galloni, G Levi, V Spalletta, G Briganti |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Cancer Research Chemotherapy education.field_of_study urogenital system medicine.medical_treatment Population nutritional and metabolic diseases Bone Marrow Stem Cell Spleen Pharmacology Biology Bleomycin Haematopoiesis chemistry.chemical_compound medicine.anatomical_structure Oncology chemistry medicine Bone marrow Stem cell education |
| Zdroj: | JNCI: Journal of the National Cancer Institute. 55:53-57 |
| ISSN: | 1460-2105 0027-8874 |
| DOI: | 10.1093/jnci/55.1.53 |
| Popis: | The mouse hematopoietic stem-cell population was tested by the spleen colony technique for effects of the antineoplastic agent bleomycin (BLM). The time response of normal bone marrow was investigated by a single dose of BLM (400 mg/kg) between 0 and 72 hours. The dose response was studied over a wide range of doses (from 40 to 1,600 mg/kg) at a 4-hour exposure. Additional experiments concerned 1) the fraction of colony-forming units in the S phase after BLM administration (by means of pulse hydroxyurea treatment), 2) the response of bone marrow stimulated by endotoxin, and 3) the effects of split-dose treatments. The relatively low toxicity of BLM on both the differentiated and stem-cell populations of unstimulated bone marrow was confirmed and detailed. This drug exhibited peculiar, proliferation-dependent cell inactivation kinetics. Furthermore, BLM induced parasynchronous behavior in the unstimulated stem-cell population. The various aspects of BLM action are discussed with regard to its use in cancer chemotherapy. |
| Databáze: | OpenAIRE |
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