Prevalence and characterisation of Salmonella Waycross and Salmonella enterica subsp. salamae in Nile perch (Lates niloticus) of Lake Victoria, Tanzania
| Autor: | Anders Dalsgaard, Barco Lisa, Lughano Kusiluka, Zebedayo Baniga, Robinson H. Mdegela |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Gill
Serotype Salmonella Veterinary medicine biology Sulfamethoxazole Nile perch 010401 analytical chemistry 04 agricultural and veterinary sciences medicine.disease_cause biology.organism_classification 040401 food science 01 natural sciences Lates 0104 chemical sciences 0404 agricultural biotechnology Salmonella enterica Ampicillin medicine Food Science Biotechnology medicine.drug |
| Zdroj: | Food Control. 100:28-34 |
| ISSN: | 0956-7135 |
| DOI: | 10.1016/j.foodcont.2019.01.006 |
| Popis: | A study was conducted to determine the prevalence, serotypes, antimicrobial resistance and plasmids profiles of Salmonella spp. in Nile perch (Lates niloticus) from Lake Victoria, Tanzania. Presence of Salmonella spp. and quantification of Escherichia coli in water and fish were investigated using standard bacteriological methods. Antimicrobial resistance was determined using minimum inhibitory concentration (MIC) method. A total of 352 samples were analysed including Nile perch, lake water and swabs from surfaces of facilities used for fish transportation. A total of 10 different Salmonella serovars were isolated. Salmonella enterica subsp. salamae with antigenic formula 42:r:- had a prevalence of 10.0% (n = 60) in Nile perch from fishing grounds which were significant different (P 0.05) compared to the ones at landing sites 10% (n = 60) and markets 13.3% (n = 60). The prevalence of S. enterica subsp. salamae 42:r:- in water from fishing grounds was 6.7% (n = 60). Two out of 12 S. enterica subsp. salamae 42:r:- were resistant to sulfamethoxazole, while one was resistance to azithromycin. S. Waycross were resistant to sulfamethoxazole (6/30), azithromycin (6/30) and ampicillin (4/30). Seven S. Waycross carried plasmids, but none of S. enterica subsp. salamae 42:r:- carried plasmids. E. coli mean counts were 2.51 log cfu/g in fish intestines, 2.38 log cfu/g in gills, and 2.02 log cfu/ml in water. The frequent findings of S. enterica subsp. salamae 42:r:- and S. Waycross in Nile perch are surprising as these serovars are rarely associated with human diseases. Follow-up studies of their ecology in Nile perch and Lake Victoria are needed. |
| Databáze: | OpenAIRE |
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