Purification and Characterization of Nitrilase Catalyzing Asymmetric Hydrolysis of Aminonitriles from Rhodococcus rhodochrous PA-34

Autor:	Ryo Muramatsu, Tek Chand Bhalla, Akiko Wakamoto, Keizo Furuhashi, Yoichi Ohba, Miho Aoshima, Akira Miura
Rok vydání:	1992
Předmět:	chemistry.chemical_classification biology Enantioselective synthesis Rhodococcus rhodochrous biology.organism_classification Nitrilase Amino acid chemistry.chemical_compound Hydrolysis chemistry Organic chemistry Propionitrile Rhodococcus Amino acid synthesis
Zdroj:	Biochemical Engineering for 2001 ISBN: 9784431681823
DOI:	10.1007/978-4-431-68180-9_61
Popis:	The α-aminonitriles are used as intermediates in the chemical synthesis of racemic amino acids and appear to be potentially attractive substrates for their hydrolysis to amino acids by microbial nitrilases. Although the production of L-alanine by Acinetobacter sp. [1] and L-phenylglycine by Aspergillus fumigatus [2] have been reported, little is known about the mechanism of enantioselective hydrolysis of α-amino-nitriles by microorganisms. In the present paper, we report the production of optically active amino acids from α-aminonitriles by Rhodococcus rhodochrous PA-34 which was isolated from soil as a propionitrile utilizing microorganism and, the purification and characterization of the nitrilase which catalyzes this asymmetric hydrolysis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::85c28c3194d1a4d1df6a5393294c9307 https://doi.org/10.1007/978-4-431-68180-9_61 Zobrazit plný text záznamu