Protein quantification by the SELDI-TOF-MS–based ProteinChip® System

Autor:	Steve Cleverley, Sonja Vorderwülbecke, Andreas Wiesner, Scot R Weinberger
Rok vydání:	2005
Předmět:	Chromatography Chemistry SELDI-TOF-MS Quantitative proteomics Cell Biology Mass spectrometry Molecular Biology Biochemistry Biotechnology
Zdroj:	Nature Methods. 2:393-395
ISSN:	1548-7105 1548-7091
DOI:	10.1038/nmeth0505-393
Popis:	Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) represents the successful combination of retentate chromatography and mass spectrometry, and this technology is an integral part of Ciphergen's ProteinChip System, which was designed to answer biomedical questions by performing protein analyses on a single experimental platform. The quantification capability of the ProteinChip System is essential in all proteomic applications for which this technology is used. Here we describe methods and results for three short experiments mimicking realistic analytical challenges to provide practical examples of quantification.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::85a616873d4421a1d2f5a4673a014b0a https://doi.org/10.1038/nmeth0505-393 Zobrazit plný text záznamu Plný text ve formátu PDF