| Autor: |
K.-F. Löhr, J.P.J. Ross |
| Rok vydání: |
1969 |
| Předmět: |
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| Zdroj: |
Research in Veterinary Science. 10:453-461 |
| ISSN: |
0034-5288 |
| DOI: |
10.1016/s0034-5288(18)34412-6 |
| Popis: |
SUMMARY An indirect fluorescent antibody test is described which detects antibodies in bovine sera against East Coast fever (Theileria parva infection). The reliability of the test was demonstrated by negative reactions with 110 scrajrom cattle of known susceptibility and high positive titres in sera from 70 cattle 1 to 4 months after experimental infection. T. parva piroplasmic and schizontal antigens were compared and the former were shown to give more accurate and consistent test readings. Cross-reactions only occurred with sera from other theilerial infections. With a T. parva antigen there was no difference in serum titres of cattle recovered from T. parva and T. lawrencci infections while those from T. mutans cases were lower. Using homologous and heterologous antigens it was possible to titrate differentially autisera from single experimental infections with T. parva and T. mutans. The test was used to measure primary antibody response in 9 survivors from experimental infection. Antibodies were first detected 8 to 14 days after initial exposure rising to peaks about the 30th day and then declining gradually but remaining detectable for at least 12 months. Following natural reinfection with T. lawrencci, responses were less marked bur conformed to the same pattern. In 15 cattle fatally infected with T. parva there was little or no antibody response. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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