Cross Species Amplification of Coat Colour Genes in Nutria (Myocastor coypus Mol)

Autor:	Tomasz Ząbek, Monika Bugno-Poniewierska, J. Bieniek, Łukasz Migdał, Stanisław Łapiński, Anna Migdał
Rok vydání:	2016
Předmět:	Genetics Coat General Medicine Biology General Biochemistry Genetics and Molecular Biology Homology (biology) chemistry.chemical_compound chemistry GenBank Mole Binding site Primer (molecular biology) Gene DNA
Zdroj:	Folia Biologica. 64:105-111
ISSN:	1734-9168 0015-5497
DOI:	10.3409/fb64_2.105
Popis:	In this study, nutria-specific DNA fragments were amplified with cross-species primers for parts of coat colour genes (MC1R, ASIP, TYRP-1, c-KIT) conserved across a number of species. Twenty-three nutria-specific DNA fragments were generated using cross-species PCR (26.7% amplification success rate). Certain limitations of this approach were encountered, including strong mismatching in the primer binding sites. Sequences of 12 nutria-specific PCR products aligned in the BLASTx option showed homology with known protein sequences. Obtained sequences were submitted to GenBank with accession number KC758969 for MC1R, KF511655 for ASIP, KF511656 for TYPR-1 and KU831489 for c-KIT. The highest homology for all translated queries was obtained with protein sequences belonging to species of the rodent family. Sequences of proteins encoded by genes involved in environmental adaptation (coat colour genes in rodents) were found to be strongly conserved among species.
Databáze:	OpenAIRE
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