The Effect of Bioactive Sutures on CD4+ I CD25+ Cells in HNSCC Cancer Patients

Autor: Vincent Y. Chen, Hausin Wong, Sanghun Kim, Kevin Nguyen, Terry Y. Shibuya, Michael Kim
Rok vydání: 2004
Předmět:
Zdroj: Otolaryngology–Head and Neck Surgery. 131
ISSN: 1097-6817
0194-5998
DOI: 10.1016/j.otohns.2004.06.170
Popis: Problem: Immunosuppression has been well recognized in head and neck squamous cell carcinoma (HNSCC). We have previously identified increased percentages of T regulator cells (T regs or CD4+/CD25+) from peripheral blood lymphocytes (PBLs) of advanced-stage HNSCC patients. T regs have been implicated in playing a very crucial role in immunosuppression. Previously we developed bioactive sutures that can enhance the immune response of cancer patients. In this study we measured the effect that stimulating HNSCC patients PBLs with bioactive sutures has on the percentage of CD4+/CD25+ T cells. Methods: A group of previously untreated advanced-stage HNSCC patients (n = 10) and controls (n = 7) were studied. PBL were drawn from patients prior to surgery and controls. Lymphocytes were purified by Ficoll-hypaque density centrifugation and stimulated in vitro with a variety of bioactive sutures (anti-CD3/28, anti-CD3/28 + IFNg, anti-CD3/28 + IL-2). The percentage of CD3/CD25, CD4/CD25, CD8/CD25, and annexin-V/propidium iodine positive cells was measured prestimulation and 6 days poststimulation. Results: HNSCC patients demonstrated 21- to 26-fold increases in CD8+/CD25+ and 7- to 8-fold increases in CD4+/CD25+ populations after suture stimulations, while controls demonstrated 36- to 40-fold increases in CD8+/CD25+ and 4-fold increases in CD4+/CD25+. Control PBLs expanded significantly higher percentages (P Conclusion: PBL from advanced-stage HNSCC patients demonstrated enhanced responses to bioactive suture stimulations, with greater expansion in the CD8+/CD25+ than CD4+/CD25+. Control PBL stimulated by bioactive sutures demonstrated even greater expansion of CD8+/CD25+ cells and less expansion of CD4+/CD25+ cells. Significance: Bioactive sutures may be useful for expanding the cytotoxic population of T cells in HNSCC patients to fight cancer. Support: California Cancer Research Program Clinician Scientist Development Award (TYS)
Databáze: OpenAIRE
Externí odkaz: