Effect of Vesicles of Dimethyldioctadecylammonium Chloride and Phospholipids on the Rate of Decarboxylation of 6-Nitrobenzisoxazole-3-carboxylate
| Autor: | Iolanda M. Cuccovia, Pedro Soares De Araujo, Maria Virgínia Scarpa, Antonio Sesso, Shirley Schreier, and Hernan Chaimovich, Anselmo Gomes de Oliveira |
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| Rok vydání: | 2000 |
| Předmět: |
chemistry.chemical_classification
Chromatography Decarboxylation Bilayer Vesicle Phospholipid Fatty acid Surfaces and Interfaces Condensed Matter Physics Trehalose chemistry.chemical_compound chemistry Phosphatidylcholine Polymer chemistry Electrochemistry lipids (amino acids peptides and proteins) General Materials Science Spectroscopy Alkyl |
| Zdroj: | Langmuir. 16:993-999 |
| ISSN: | 1520-5827 0743-7463 |
| DOI: | 10.1021/la9907477 |
| Popis: | Sonicated mixtures of dimethyldioctadecylammonium chloride (DODAC), egg phosphatidylcholine (PC), dimyristoyl phosphatidylcholine (DMPC), and dipalmitoyl phosphatidylcholine (DPPC) were used to analyze vesicle effects on the rate of decarboxylation of 6-nitrobenzisoxazol-3-carboxylic acid (Nboc). Electron microscopic images of the vesicles were obtained with trehalose, a know cryoprotector. Phase diagrams and phase transitions temperatures of the vesicle bilayers were determined. Nboc decarboxylation rates increased in the presence of vesicles prepared with both phospholipids and DODAC/phospholipid mixtures. Quantitative analysis of vesicular effects was done using pseudophase models. Phospholipids catalyzed up to 140-fold while the maximum catalysis by DODAC/lipid vesicles reached 800-fold. Acceleration depends on alkyl chain length, fatty acid insaturation of the lipids, and the DODAC/phospholipid molar ratio. Catalysis is not related to the liquid crystalline-gel state of the bilayer and may be related... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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