Impairment of caprine oocyte maturation in vitro and alteration of granulosa cells functions by widely used fungicide mancozeb

Autor:	Suranga P. Kodithuwakku, Madhusha Prasadani, Sandarenu Kodikara, B. Alexander, Indunil Pathirana, Kai-Fai Lee, Chathura Rathnayake, Ishani Dinisri
Rok vydání:	2021
Předmět:	endocrine system urogenital system Chemistry Granulosa cell media_common.quotation_subject Progesterone secretion Oocyte Cell morphology Andrology medicine.anatomical_structure Human fertilization Food Animals medicine Animal Science and Zoology Mancozeb Viability assay Ovulation media_common
Zdroj:	Tropical Animal Health and Production. 53
ISSN:	1573-7438 0049-4747
DOI:	10.1007/s11250-021-02854-5
Popis:	Mancozeb is classified as an endocrine disruptor; thus the present study was carried out to investigate the impact of mancozeb on mammalian ovarian functions using in vitro caprine oocyte maturation and granulosa cell culture models. Caprine cumulus oocyte complexes (COCs) and granulosa cells were cultured under standard culture conditions and treated with mancozeb concentrations of 0.3, 3, and 30 μg/ml along with a control for 24 h and assessed. Granulosa cell viability and progesterone concentration in spent culture media after treatments were also assessed. Mancozeb significantly decreased (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::855bc110eadd4b01e8a5f65634c2c863 https://doi.org/10.1007/s11250-021-02854-5 Zobrazit plný text záznamu Full text from SpringerLink