Characterization and infectivity of a spontaneous variant isolated from Frankia sp. WEY 0131391

Autor: Linda R. Robertson, Carolyn V. Carpenter, Willie L. Parson
Rok vydání: 1985
Předmět:
Zdroj: Frankia and Actinorhizal Plants ISBN: 9789401087773
DOI: 10.1007/978-94-009-5147-1_3
Popis: A spontaneous variant, obtained from a Frankia isolate from Alnus rubra nodules, was compared with the parent strain with regard to infeetivity, nitrogenase activity, and electro- phoretic and immunological profiles. Both the parent and the variant strain were equally effective in inducing nodulation in seedlings of A. rubra. All inoculated plants had an active nitrogenase system as measured by the acetylene reduction assay. Electrophoresis of whole cell homogenates on SDS-polyacrylamide slab gels showed similar electrophoretic profiles; however, the variant strain also exhibited striking differences in protein patterns that distinguish it from the parent strain. Immunological analysis of the original Frankia strain and its variant revealed shared antigens as well as immunologically distinct antigenic determinants in the two strains. The variant strain exhibits a distinct morphology and growth patterns which remain stable after many passages through culture.
Databáze: OpenAIRE