Expression of anti-Müllerian hormone in two rat models of polycystic ovary syndrome
| Autor: | Dan-Feng Du, Sai-Hua Zheng, Xue-Lian Li |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
endocrine system
medicine.medical_specialty endocrine system diseases 030209 endocrinology & metabolism Prasterone sulfate Human chorionic gonadotropin Pathogenesis 03 medical and health sciences 0302 clinical medicine Internal medicine Medicine Levonorgestrel 030219 obstetrics & reproductive medicine biology urogenital system business.industry Obstetrics and Gynecology Anti-Müllerian hormone Silastic Polycystic ovary female genital diseases and pregnancy complications Endocrinology biology.protein business hormones hormone substitutes and hormone antagonists medicine.drug Hormone |
| Zdroj: | Journal of Obstetrics and Gynaecology Research. 42:1761-1767 |
| ISSN: | 1341-8076 |
| DOI: | 10.1111/jog.13118 |
| Popis: | Aim Anti-Mullerian hormone (AMH) levels are two to three times higher in patients with polycystic ovary syndrome (PCOS), but the mechanism of increased AMH levels in PCOS remains unclear. The purpose of our experiment was to investigate a change in AMH levels in two kinds of commonly used rat models and to determine an ideal model for future research of AMH in the pathogenesis of PCOS. Methods Thirty female Sprague Dawley rats were treated using two modeling methods: implantation of a levonorgestrel silastic implant or injection with sodium prasterone sulfate plus human chorionic gonadotropin (hCG). Rats in the control group were implanted with a blank silastic stick. Serum steroid concentrations, ovarian morphology and ovarian expression of AMH and AMH-receptor II (RII) proteins were determined and their correlations were studied. Results The results from the levonorgestrel and hCG group were closer to those displayed by human PCOS patients than the sodium prasterone sulfate and hCG group. Ovarian local expression of AMH and AMH-RII was increased in these both models compared with the control group; however, an elevation of serum AMH concentration was not observed (12.53 ± 0.99 ng/ml and 13.22 ± 1.09 ng/ml vs 16.30 ± 0.98 ng/ml). Conclusion The levonorgestrel and hCG model is more suitable for the study of PCOS in puberty. |
| Databáze: | OpenAIRE |
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