Functional characterization of podia formation in normal and malignant hematopoietic cells

Autor:	Julian Topaly, K. Srbic, A. D. Ho, Stefan Fruehauf, R. Seggewiss
Rok vydání:	2002
Předmět:	Immunology CD34 Video microscopy Cell migration Cell Biology Biology CXCR4 Cell biology Haematopoiesis medicine.anatomical_structure medicine Immunology and Allergy Bone marrow Kinase activity Stem cell
Zdroj:	Journal of Leukocyte Biology. 71:425-432
ISSN:	1938-3673 0741-5400
DOI:	10.1189/jlb.71.3.425
Popis:	Hematopoietic cells extend multiple podia of yet unknown function. Our morphological studies using scanning electron microscopy and functional studies using time-lapse video microscopy suggest that podia formed by CD34+ hematopoietic stem cells (HSC) on the bone marrow stroma component fibronectin are characteristic of lamellipodia at the leading edge and uropodia at the trailing edge, cytoskeletal structures that have previously been shown to be responsible for cell locomotion of lymphocytes. In the leukemic cells studied here, stroma-derived factor-1α (SDF-1α) led to a significant eightfold increase in transmigration (BCR-ABL-positive BV173 leukemia cell line;P
Databáze:	OpenAIRE
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