Abstract 2631: Analysis of PI3K pathway genes in bladder cancer
| Autor: | Didem Seven, Hatice Tigli, Nur Buyru |
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| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Cancer Research. 72:2631-2631 |
| ISSN: | 1538-7445 0008-5472 |
| DOI: | 10.1158/1538-7445.am2012-2631 |
| Popis: | Bladder cancer is the most common cancer of the urinary tract and is the ninth the most common cancer among men. Its incidence directly increases with age, it rarely occurs before the age of 50 and it is three to four times more common in men compared to women. Although there is now exclusive information on bladder cancer genetics, epigenetics and gene expression, several questions remain unanswered. Global profiling via expression and genomic microarray technologies may provide answers to some of the outstanding questions. Many studies have assessed the status of known oncogenes and tumor suppressor genes and have investigated common chromosomal alterations to identify novel genomic events in bladder cancer. The phosphatidylinositol 3-kinase (PI3K) signaling pathway plays critical role in cell growth, proliferation, and apoptosis. As a result of genomic alterations and loss of some tumor suppressor genes signaling through this pathway is commonly altered in many types of cancer including bladder. In the present study, our interest was focused on the expression analysis of some critical genes in this pathway. For this purpose, expression of seven genes (mTOR, KRAS, PIK3CA, TSC1, STK11, Rheb, PTEN and AKT1) acting in this pathway was analyzed in 35 matched bladder tumor and non-cancerous tissue samples by quantitative RT-PCR. Expression of all genes except PIK3CA and PTEN were similar for the normal bladder and tumor tissue. We observed downregulated expression of PTEN and PIK3CA genes in bladder tumors. The median PTEN mRNA levels in non-cancerous and tumor tissue samples were 2.11 and 0.35, respectively and the difference between the two groups was statistically significant (p=0.00). PIK3CA was also downregulated in the tumor tissues. Tumor tissue samples displayed 6.62 fold decrease in PIK3CA expression compared to normal tissue samples (p=0.002). On the other hand, slightly decreased Rheb mRNA expression was also observed in tumor tissue compared to the non-cancerous tissue. However, the difference expression levels of Rheb between two groups was not statistically significant. Our result indicate that, PI3K pathway has an important function in bladder cancer progression with the contribution of more than one gene in this pathway. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2631. doi:1538-7445.AM2012-2631 |
| Databáze: | OpenAIRE |
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