Enzyme immobilization on smart polymers: Catalysis on demand
Autor: | Manuela Curcio, Nevio Picci, Giuseppe Cirillo, Umile Gianfranco Spizzirri, Francesca Iemma, Fiore Pasquale Nicoletta |
---|---|
Rok vydání: | 2014 |
Předmět: |
Polymers and Plastics
Immobilized enzyme Chemistry General Chemical Engineering Radical polymerization technology industry and agriculture Substrate (chemistry) General Chemistry Biochemistry Smart polymer chemistry.chemical_compound Monomer Self-healing hydrogels Polymer chemistry Materials Chemistry Environmental Chemistry Thermal stability Ethylene glycol Nuclear chemistry |
Zdroj: | Reactive and Functional Polymers. 83:62-69 |
ISSN: | 1381-5148 |
Popis: | A new approach for the synthesis of hydrogel films with thermo-sensitive enzymatic activity is reported. Pepsin (PEP) was covalently immobilized on thermo-responsive hydrogels by radical polymerization in the presence of N-isopropylacrylamide and poly-(ethylene glycol) dimethacrylate 750, acting as functional monomer and crosslinking agent, respectively. Hydrogels showing lower critical solution temperatures between 32.9 and 36.1 °C were synthesized by UV-irradiation of reaction batches differing in the PEP/monomers ratio. The derivatization degree of the hydrogels was expressed as mg of PEP per gram of matrix and found to be in the range of 6 to 11% as assessed by Lowry method. Scanning electron microscopy analysis and water affinity evaluation allowed to highlight the porous morphology and thermo-responsivity of hydrogels as a function of temperature. Using bovine serum albumin as a substrate, kinetics parameters were determined by Lineweaver–Burk plots and the catalyst efficiency evaluated. The influence of temperature on enzyme activity, as well as the thermal stability and reusability of devices, were also investigated. |
Databáze: | OpenAIRE |
Externí odkaz: |