CD56 identifies monocytes and not natural killer cells in rhesus macaques

Autor: R.L. Blosser, T.M. Shieh, Karen Chadwick, Joseph B. Margolick, M C Zink, James E. K. Hildreth, D.L. Carter, Janice E. Clements
Rok vydání: 1999
Předmět:
Zdroj: Cytometry. 37:41-50
ISSN: 1097-0320
0196-4763
DOI: 10.1002/(sici)1097-0320(19990901)37:1<41::aid-cyto5>3.0.co;2-4
Popis: Background: CD56 is a lineage-specific marker of human natural killer (NK) cells. There are conflicts in the literature regarding the role of CD56 as a marker of NK cells in non-human primates. In the present study, we examined the role of CD56 in identifying rhesus NK cells. Methods: The immunophenotype of normal macaque and human NK cells was analyzed by two- and three-color flow cytometry. Flow cytometric cell sorting was subsequently used to deplete or purify NK cells; the resulting cell populations were then used in standard chromium release assays of NK lytic function. Results: In peripheral blood mononuclear cells of the rhesus macaque, CD56 was expressed primarily on cells with the light scatter and immunophenotypic profile of monocytes. Flow cytometric depletion of rhesus CD56+ monocytic cells did not diminish functional activity against K562 cells, whereas depletion of CD8+ or CD16+ lymphocytes completely abrogated functional activity. Three-color flow cytometric analysis of CD8+, CD16+ lymphocytes showed that they expressed other markers (CD2, CD7, TIA-1) associated with NK cells, but notably, not CD56. Conclusions: These studies demonstrate that CD56 is not suitable as a marker of NK cells in the rhesus macaque. Cytometry 37:41–50, 1999. © 1999 Wiley-Liss, Inc.
Databáze: OpenAIRE
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