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Botrytis species include some serious fungal plant pathogens, which are implicated in many diseases affecting flowers, fruits, cereals, legumes, and other vegetables. In particular, Botrytis cinerea attacks economically important crops such as carrots, grapes, lettuce, strawberries, and tobacco, producing various leaf spot diseases and grey mould. In this work, we have studied, in vitro, the growth and the sporulation of some isolates of Botrytis cinerea in different nutrient media. Our aim was to select the isolate the most pathogen to use it in biological control tests. For this purpose, ten isolates of Botrytis cinerea were isolated, purified and identified in the laboratory. Seven isolates were isolated from samples of strawberry fruit harvested from fields; four of them, are originated from strawberry fields of Zlaoula and Laâwamra (Larache) and three are originated from fields of Moulay Bouselham (Algharb). Others are isolated from postharvest strawberry. The growth and sporulation of all of the isolates are studied and compared in different nutrient media PDA, MEA, Czapek and organic medium of strawberry. Among all of the isolates tested, Botrytis cinerea Bt7 originated from fields of Zlaoula was the most important isolate with maximum growth in all of nutrients media, and maximum sporulation (74,7.105 sp/ml). B. cinerea was inoculated artificially in leaf and fruit stawberry and it causes the visible disease symptoms of grey mould. |
