Isolation and characterization of antineoplastic alkaloids from Catharanthus roseus l. Don. cultivated in Egypt
| Autor: | Mostafa M El-Missiry, Khaled A. Shams, Medhat M Seif El Nasr, Naglaa M Nazif, Nahla S. Abdel Azim, Khaled A Abdel Shafeek, S. I. Ismail |
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| Rok vydání: | 2010 |
| Předmět: |
Chromatography
biology Chemistry Silica gel Plant composition Catharanthus roseus Isolation (microbiology) biology.organism_classification High-performance liquid chromatography Radial chromatography chemistry.chemical_compound Complementary and alternative medicine Drug Discovery Chromatographic column |
| Zdroj: | African Journal of Traditional, Complementary and Alternative Medicines. 6 |
| ISSN: | 0189-6016 |
| DOI: | 10.4314/ajtcam.v6i2.57082 |
| Popis: | Vinblastine and vincristine (the antileukemic agents) were isolated, in a pure form, from Catharanthus roseus L. Don., cultivated in Egypt, by several chromatographic techniques. Five modified methods for the preparation of total alkaloids were carried out. All the isolated mixtures were evaluated by HPLC and HPTLC analyses. The antineoplastic alkaloids; vinblastine and vincristine, were isolated by the use of vacuum liquid chromatographic column on silica gel : aluminium oxide (1:1) mixed bed vacuum liquid chromatography (VLC), Charcoal column, and finally purified by centrifugally accelerated radial chromatography (Chromatotrone). |
| Databáze: | OpenAIRE |
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