The Use of Porcine Proximal Tubular Cells for Studying Nephrotoxicity In Vitro: Validation of the Effects of Culturing and Cryopreservation
| Autor: | Marieke Kruidering, J. F. Nagelkerke, E. de Heer, D. H. Maasdam, W.R. Leeman |
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| Rok vydání: | 1994 |
| Předmět: |
0301 basic medicine
Kidney medicine.medical_specialty Renal tubule 030102 biochemistry & molecular biology medicine.diagnostic_test Chemistry General Medicine Toxicology General Biochemistry Genetics and Molecular Biology In vitro Cryopreservation Nephrotoxicity Cell biology Flow cytometry 03 medical and health sciences Medical Laboratory Technology 0302 clinical medicine medicine.anatomical_structure Investigation methods Endocrinology 030220 oncology & carcinogenesis Internal medicine medicine Proximal tubule |
| Zdroj: | Alternatives to Laboratory Animals. 22:462-473 |
| ISSN: | 2632-3559 0261-1929 |
| Popis: | The susceptibility to nephrotoxins of freshly isolated porcine proximal tubular cells (PPTC) and cultured PPTC in suspension were compared, with a view to using PPTC as in vitro models for studying nephrotoxicity. In a previous paper, we described how, in freshly isolated PPTC, well-known nephrotoxins such as mercury (II) chloride, cisplatin, p-aminophenol and halogenated hydrocarbons caused a dose-dependent decrease in the viability and mitochondrial membrane potential of the PPTC. In this paper, we show that suspensions of cultured PPTC, harvested by trypsinisation, are less susceptible to nephrotoxins, possibly due to the synthesis of extracellular matrix components. PPTC in primary culture are suitable for relatively long-term nephrotoxicity studies. This was demonstrated by incubation with mercury (II) chloride for 24 hours, resulting in a dose-dependent loss of viability. Freshly isolated PPTC can be cryopreserved by computer-controlled freezing. The cryopreserved PPTC displayed an increased susceptibility to mercury (II) chloride and a decreased susceptibility to cisplatin and 1,1-dichloro-2,2-difluoroethylene-cysteine, the toxicity of the latter indicating that the renal enzyme β-lyase remains active during cryopreservation. |
| Databáze: | OpenAIRE |
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