| Autor: |
Leonard D. Stuart, H. Hugh Fudenberg, Eugenia Floyd, Gary V. Paddock, Gregory B. Wilson, Martin L. Morin, Amanda M. Williams, Louise Just |
| Rok vydání: |
1983 |
| Předmět: |
|
| DOI: |
10.1016/b978-0-12-409850-3.50022-x |
| Popis: |
Publisher Summary The dialyzable leukocyte extracts (DLE) prepared from burros immunized with either Mycobacterium tuberculosis (PPD–S) or Coccidioides immitis (spherulin) antigen can transfer antigen-specific cell-mediated immunity (CMI) to previously nonresponsive human lymphocytes in vitro. The results of experiments described in this chapter indicate that when Burros are immunized with either Mycobacterium tuberculosis antigen or Coccidioides immitis antigen, DLE subsequently prepared from their PBL contains TF activity, which is active in inducing antigen-specific responsiveness in previously nonimmune human lymphocytes. Burro TF activity easily can be detected using the LMI assay, the in vitro assay for human TF activity. Characterization of Burro TP specific for PPD–S indicates that it has physical–chemical properties similar to those of human TF derived from DLE. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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