Identification and natural functions of cyclic lipopeptides fromBacillus amyloliquefaciensAn6
| Autor: | Moncef Nasri, Philippe Jacques, Max Béchet, Hanen Ben Ayed, Noomen Hmidet |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Environmental Engineering Strain (chemistry) Bacillus amyloliquefaciens biology Proteolytic enzymes Bioengineering biology.organism_classification Antimicrobial 01 natural sciences In vitro Microbiology 03 medical and health sciences Bacillomycin chemistry.chemical_compound 030104 developmental biology chemistry 010608 biotechnology Surfactin Bacteria Biotechnology |
| Zdroj: | Engineering in Life Sciences. 17:536-544 |
| ISSN: | 1618-0240 |
| Popis: | Lipopeptides constitute a structurally diverse group of metabolites produced by various bacterial and fungal genera. In the past decades, research on lipopeptides has been fueled by their surfactant activities. However, natural functions of lipopeptides compounds have received considerably less attention. The aim of this study was to isolate and identify the lipopeptides from Bacillus amyloliquefaciens An6, and further evaluate their biological activities. An6 lipopeptides were detected by PCR using degenerated primers and MALDI-TOF-MS. An6 strain was found to produce surfactin, fengycin, and bacillomycin. Following their purification, the in vitro antioxidant activity of An6 lipopeptides was studied through different assays. The scavenging effect on 1,1-diphenyl-2-picrylhydrazyl radicals at a dosage of 0.75 mg/mL was 81%. Its reducing power was concentration-dependant and reached a maximum of 1.07 at 2.5 mg/mL. Moreover, they showed a strong inhibition of β-carotene bleaching. An6 lipopeptides mixture was also found to display significant antimicrobial activity against several Gram-positive, Gram-negative bacteria, and fungal strains. An6 lipopeptides were insensitive to proteolytic enzymes, stable between pH 4.0 and 12.0, and resistant to high temperature. Our results provided enough evidence proving that An6 lipopeptides could be used as functional-food components. |
| Databáze: | OpenAIRE |
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