Influence of production media on Cinchona cell cultures; spontaneous formation of β-carbolines from L-tryptophan

Autor:	Robert Verpoorte, T. van der Leer, R. Wijnsma, P.J. De Jong, P. A. A. Harkes
Rok vydání:	1986
Předmět:	biology Cinchona ledgeriana Alkaloid Quinoline Tryptophan Cinchona Plant Science General Medicine biology.organism_classification chemistry.chemical_compound Tissue culture chemistry Biotransformation Biochemistry Cell culture Genetics Agronomy and Crop Science
Zdroj:	Plant Science. 47:71-76
ISSN:	0168-9452
DOI:	10.1016/0168-9452(86)90012-9
Popis:	Evidence is presented that the β-carboline alkaloids norharman and harman are artifacts formed from L-tryptophan. Transfer of Cinchona ledgeriana suspension cultured cells to Zenk's alkaloid production medium (ZAP medium) resulted in cell death. No quinoline or terpenoid indole alkaloids were formed. However, the L-tryptophan present in the production medium was transformed to the β-carboline alkaloids, harman and norharman. It was demonstrated that norharman was also formed in ZAP medium without cells and in ZAP medium containing frost-killed cell material.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::84b611c9a5f1e6cdfa9ad4444b6e5cfd https://doi.org/10.1016/0168-9452(86)90012-9 Zobrazit plný text záznamu