Enzymatic propyl gallate synthesis in solvent-free system: Optimization by response surface methodology
| Autor: | Habib Horchani, Youssef Gargouri, Ali Chaari, Nadia Ben Salem, Ahlem Bouaziz, Moncef Chaabouni, Adel Sayari |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Antioxidant
biology Chemistry Process Chemistry and Technology medicine.medical_treatment Staphylococcus xylosus Bioengineering biology.organism_classification Ascorbic acid Biochemistry Catalysis Propanol chemistry.chemical_compound Yield (chemistry) biology.protein medicine Organic chemistry Gallic acid Lipase Propyl gallate Nuclear chemistry |
| Zdroj: | Journal of Molecular Catalysis B: Enzymatic. 67:242-250 |
| ISSN: | 1381-1177 |
| DOI: | 10.1016/j.molcatb.2010.08.013 |
| Popis: | The ability of a non-commercial immobilized Staphylococcus xylosus lipase to catalyze the esterification of propanol with gallic acid was investigated and the antioxidant as well as the antimicrobial activities of the ester formed were evaluated. The response surface methodology, based on a three variables Box–Behnken design (reaction temperature, enzyme amount and 1-propanol/gallic acid molar ratio), was used to optimize the experimental conditions of propylgallate synthesis. The maximum conversion yield (90% ±3.5) was obtained by using 400 IU of immobilized lipase and a propanol/gallic acid at a molar ratio of 160 at 52 ◦ C. The obtained ester was characterized by spectroscopic methods, NMR and FTIR. The antioxidant activity of propyl gallate was evaluated and compared to the synthetic classical antioxidants, BHA and ascorbic acid, taken as references. In addition, the antimicrobial activity of the propyl gallate was tested against S. xylosus, Escherchia coli and Staphylococcus aureus using disc diffusion and macrodilution methods. Our results show that the synthesized propyl gallate ester presents a higher antioxidant and antimicrobial power than the parent gallic acid as well as the synthetic classical antioxidants. © 2010 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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