A novel fluorescent turn-on probe for highly selective detection of nitroreductase in tumor cells
| Autor: | Lei Wang, Tianyi Qin, Kangning Zhu, Chen Zhao, Yingying Huang, Zijie Luo, Bin Liu |
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| Rok vydání: | 2018 |
| Předmět: |
Detection limit
Chalcone Biocompatibility Metals and Alloys Tumor cells 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Highly selective 01 natural sciences Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Turn (biochemistry) chemistry.chemical_compound Nitroreductase chemistry Materials Chemistry Biophysics Electrical and Electronic Engineering 0210 nano-technology Instrumentation |
| Zdroj: | Sensors and Actuators B: Chemical. 276:397-403 |
| ISSN: | 0925-4005 |
| Popis: | The expression level of nitroreductase (NTR) is directly associated with cellular hypoxia states, which plays an important role in cancer invasion, metastasis, and resistance to treatment. Therefore, developing a facile and effective method for NTR detection is of great importance for improving anticancer treatment efficacies. To date, fluorescence method has received extensive attention for NTR detection due to its non-invasion, high sensitivity, and high spatial-temporal resolution features. However, most of fluorescent probes suffered from sophisticated systhesis procedure and poor biocompatibility, which would severely limit their practical applications especially for bioimaging. Herein, we repoted a novel chalcone-based fluorescence probe with simple preparation procedure and excellent biocompatibility for detection of NTR. The probe exhibits a highly selective fluorescence response towards NTR and the detection limit reaches 27 ng/mL. The specific fluorescence response can be ascribed to the NTR-catalyzed nitrobenzyl reduction mechanism, which has been verified by using fluorescence spectra, fluorescence lifetime, mass spectra, and inhibition experiments. Notably, this method successfully achieves hypoxia imaging in tumor cells, providing a useful tool for hypoxia pre-diagnosis. |
| Databáze: | OpenAIRE |
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