Detection of nongroup A rotaviruses in faecal samples of pigs in the Czech Republic
| Autor: | Smíd B, Rodák L, I. Psikal, R. Smitalova |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
0301 basic medicine
General Veterinary 040301 veterinary sciences viruses Pcr cloning Nucleic acid sequence Prevalence virus diseases 04 agricultural and veterinary sciences Biology medicine.disease_cause Virology Group A Group B Microbiology law.invention 0403 veterinary science 03 medical and health sciences fluids and secretions 030104 developmental biology law Rotavirus medicine Polymerase chain reaction Feces |
| Zdroj: | Veterinární medicína. 54:12-18 |
| ISSN: | 1805-9392 0375-8427 |
| DOI: | 10.17221/3081-vetmed |
| Popis: | Besides group A rotaviruses, group B and C rotaviruses have been detected as the cause of diarrheal diseases in pigs. Of a set of 329 faecal samples from pigs, 16 samples were selected in which rotavirus was detected by electron microscopy and at the same time group A rotavirus was excluded by ELISA method. Rotaviruses were assayed using specific primers for detection of group B and C rotaviruses, and RT-PCR and semi-nested PCR methods. In one sample, no rotavirus of group B or C was detected; in the remaining 15 samples rotavirus group C was detected, in two samples together with group B rotavirus. Sequencing of the obtained PCR products and comparison with corresponding gene sequences revealed 80% nucleotide sequence identity between group B rota- viruses and available sequences of porcine isolates. A nucleotide sequence identity of 92% was obtained in group C rotaviruses as compared with the Cowden strain. |
| Databáze: | OpenAIRE |
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