| Popis: |
The possible protective effects of n hexane extract Acrocarpus fraxinifolius leaves (nHEAFL) were assessed against the APAP induced organ toxicity in male rats. Also, the content of polyphenols extracted from AFL was studied, and their relationship with antioxidant activity was investigated. nHEAFL was tested for cytotoxicity on Vero cell line, with reference to IC50, and other non toxic concentrations of all the extracts. The antiviral activity against HSV1 for all non toxic concentrations of the extract was determined using plaque reduction assay. It was found that nHEAF showed a reduction of serum hepatic/renal cellular toxicity and cellular lipid peroxidation in kidney, spleen & heart, as well as enhanced cellular antioxidant defense system in tissues. Also, our results revealed that the inhibitory activity of the HSV1 virus was dose dependent on the polyphenol content of the examined extract. The minimum inhibitory concentration (MIC) for the nHEAF extract was determined as well as the EC50 and SI. Calculated SI showed promising value for the nHEAFL, and hence can be used as therapeutic medication for HSV1. Direct contact between the HSV1 and the examined extract in cell free assay system showed different degrees of virucidal activity depending on the polyphenolic content of these extracts. In order to study other possible mode of action, Vero cells were treated with the examined extracts before, during, and after virus infection to give an insight on the interference of the extract in each step in the virus life cycle. In conclusion, nHEAFL showed a remarkable organs protective effect against APAP induced organs toxicity in rats. Also, examined extracts exhibited the antiviral activity against HSV1 via blocking of the virus attachment and penetration and inhibition of the early stage of viral replications. |
