Chronic deletion, escape from deletion and activation of mouse mammary tumor virus superantigen-reactive T cells in C57BL/10 mice
| Autor: | P. Julian Dyson, James I. Elliott |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
chemistry.chemical_classification
biology Immunology Cell Mouse mammary tumor virus T-cell receptor chemical and pharmacologic phenomena Endogeny Peptide Major histocompatibility complex biology.organism_classification Cell biology medicine.anatomical_structure chemistry parasitic diseases T cell selection biology.protein medicine Superantigen Immunology and Allergy |
| Zdroj: | European Journal of Immunology. 29:1456-1466 |
| ISSN: | 1521-4141 0014-2980 |
| Popis: | Though C57BL/10 mice express the mouse mammary tumor virus superantigens (sag) encoded by Mtv-8 and Mtv-9, it has been thought that these sag do not bind to the MHC class II molecule H2-Ab and consequently do not affect the T cell repertoire. However, we show that cells bearing TCR Vbeta chains specific for Mtv-8 and -9 sag are chronically deleted in C57BL/10 mice. Thymocytes and peripheral T cells escaping deletion by Mtv sag display a small reduction in the level of cell surface CD4. T cells escaping thymic deletion respond variably to endogenous Mtv sag with some, but not all, reactive populations appearing overrepresented in the activated/memory subset. The data suggest that in normal mice fine modulation of coreceptor expression levels may be a common way by which thymocytes escape elimination, that systems utilizing potentially Mtv sag-reactive TCR on a C57BL background may be inappropriate for the measurement of the affinity of TCR/MHC/peptide interactions required in thymic selection, and that detection of the activity of human sag may be aided by analysis of CD4 levels and activation markers on T cells in conjunction with studies of the frequency of cells bearing specific TCRVbeta chains. |
| Databáze: | OpenAIRE |
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