How To Determine Diffusion Coefficients in Planar Phospholipid Systems by Confocal Fluorescence Correlation Spectroscopy
| Autor: | Martin Hof, A. Benda, A. Lhotský, and W. Th. Hermens, V. Mareček, M. Beneš |
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| Rok vydání: | 2003 |
| Předmět: |
Confocal
Analytical chemistry Fluorescence correlation spectroscopy Surfaces and Interfaces Condensed Matter Physics Laser Fluorescence law.invention Rhodamine chemistry.chemical_compound chemistry law Electrochemistry General Materials Science Fluorescence cross-correlation spectroscopy Mica Diffusion (business) Spectroscopy |
| Zdroj: | Langmuir. 19:4120-4126 |
| ISSN: | 1520-5827 0743-7463 |
| DOI: | 10.1021/la0270136 |
| Popis: | Confocal fluorescence correlation spectroscopy (FCS) allows for the determination of lateral diffusion coefficients and surface densities in planar phospholipid systems. The determination of the vertical (z-) position of the laser focus relative to the phospholipid surface plane is of crucial importance for the accuracy of the confocal FCS experiment. In this work we determine for the first time this vertical (z-) position of the laser focus by a so-called “Z-scan”, which is based on the determination of diffusion times and particle numbers in 0.2 μm steps along the vertical (z-) axis. Experiments on supported phospholipid bilayers composed of dioleoylphosphatidylcholine (DOPC) and small amounts of Rhodamine Red-X 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Rhodamine Red-X DHPE) adsorbed onto atomically flat mica and borosilicate glass demonstrate that results obtained by the Z-scan approach are significantly more precise than those results obtained when the fluorescence in... |
| Databáze: | OpenAIRE |
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