| Autor: |
Leah R. Padgett, Delini K. Samarasinghe, Yuan-Po Tu, Lauren A. Kennington, Michelle L. Wallander, Charlotte L. Ahls, Douglas Rains, James S. Elliott |
| Rok vydání: |
2020 |
| Předmět: |
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| DOI: |
10.1101/2020.10.09.20210302 |
| Popis: |
BackgroundPolyester nasal swabs stored in saline or in a dry tube were evaluated as an alternative to foam nasal swabs for SARS-CoV-2 testing by reverse transcription quantitative polymerase chain reaction (RT-qPCR) since they may be inexpensively manufactured at high capacity.MethodsSurrogate clinical specimens were prepared by inoculating foam and polyester nasal swabs with residual SARS-CoV-2 positive clinical specimens diluted in porcine or human matrix. Dry swab elution with phosphate buffered saline (PBS) was evaluated by vortex, swab swirling, and passive methodologies. Surrogate and clinical nasal specimen stability were evaluated at refrigerated (4°C) and elevated temperatures (40°C for 12 hours, 32°C hold) through 72 hours.ResultsPolyester swabs demonstrated equivalent performance to foam swabs for detection of low and high SARS-CoV-2 viral loads. Dry swab elution performed with PBS and mechanical disruption by vortex resulted in nearly complete quantitative recovery of virus. Dry polyester and foam surrogate specimens were stable through 72 hours both when refrigerated and after high temperature excursion, which simulated specimen transport without cold chain. Similarly, clinical specimens collected with polyester swabs and stored dry were stable through 72 hours in the presence and absence of cold chain. Polyester surrogate specimens stored in saline were stable through 72 hours refrigerated but only through 48 hours at elevated temperatures.ConclusionsPolyester nasal swabs stored in dry collection tubes comprise a robust and inexpensive self-collection method for SARS-CoV-2 viral load testing, which is stable under conditions required for home collection and shipment to the laboratory. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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