A hybrid two-component system of Tannerella forsythia affects autoaggregation and post-translational modification of surface proteins

Autor:	Hiroshi Nakamura, Daisuke Niwa, Kiyoshi Nishikawa
Rok vydání:	2011
Předmět:	Gel electrophoresis biology Mutant biology.organism_classification Microbiology Molecular biology Two-component regulatory system Response regulator Forsythia Proteome Gene cluster Genetics Tannerella forsythia Molecular Biology
Zdroj:	FEMS Microbiology Letters. 318:189-196
ISSN:	0378-1097
DOI:	10.1111/j.1574-6968.2011.02256.x
Popis:	Tannerella forsythia is a Gram-negative oral anaerobe closely associated with both periodontal and periapical diseases. The ORF TF0022 of strain ATCC 43037 encodes a hybrid two-component system consisting of an N-terminal histidine kinase and a C-terminal response regulator. Disruption of the TF0022 locus enhanced autoaggregation of the broth-cultured cells. Comparative proteome analyses revealed that two S-layer proteins in the TF0022 mutant exhibited decreased apparent masses by denaturing gel electrophoresis, suggesting a deficiency in post-translational modification. Furthermore, the mutant decreased the production of a glycosyltransferase encoded by TF1061 that is located in a putative glycosylation-related gene cluster. Quantitative real-time PCR revealed reduced transcription of TF1061 and the associated genes in the TF0022 mutant. These results indicate that TF0022 upregulates the expression of the glycosylation-related genes and suggest modulation of the autoaggregation of T. forsythia cells by a possible post-translational modification of cell-surface components.
Databáze:	OpenAIRE
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