| Autor: |
Mingjun Xiang, Lin Zhu, Siyuan Wang, Shiqiang He, Zhiliang Zhang, Siqi Xiang, Ruxia Cui |
| Rok vydání: |
2020 |
| Předmět: |
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| DOI: |
10.21203/rs.3.rs-95381/v1 |
| Popis: |
Backgrounds Although inhibitor of apoptosis protein-like protein-2 (ILP-2) is regarded as a novel growth enhancer for breast cancer, its mechanism on breast cancer cell growth remains elusive. This study analysed the expression profiles of proteins with association to ILP-2 protein during Michigan Cancer Foundation-7 (MCF-7) breast cell growth for its mechanism on breast cancer cell growth.Methods Isobaric tags for relative and absolute quantification analysis (iTRAQ) was applied on siRNA-5 and negative control groups of MCF-7 breast cancer cells to analyse protein expression profile correlated to ILP-2 during MCF-7 cell growth. Verification of iTRAQ data was by done by Western blot.Results4065 proteins were identified in the MCF-7 cells, with 241 of differentially expressed proteins (DEPs) (fold change ≥ 1.20 or ≤ 0.83 and PConclusionsThese results suggest that ILP-2 promotes MCF-7 cell growth by regulating cell proliferation, signal transduction, and immune system processes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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