Characterization of the diffusion of fluorophores within microbial biofilms by flourescence correlation microscopy under two-photon excitation

Autor: B. Arrio, P. Georges, A. Brun, Fabienne Merola, M. P. Fontaine-Aupart, E. Guiot, R. Briandet, Marie-Noëlle Bellon-Fontaine
Rok vydání: 2001
Předmět:
Zdroj: Photon Migration, Optical Coherence Tomography, and Microscopy.
ISSN: 0277-786X
DOI: 10.1117/12.447419
Popis: The penetration and diffusion capabilities of fluorophores inside microbial biofilms of heterogeneous three-dimensional structure were studied by fluorescence correlation microscopy under two-photon excitation. The influence of the size and the surface charge of the fluorophores were investigated in order to understand the mechanisms involved in molecular diffusion inhibition occuring in biofilms such as electrostatic and steric interactions.© (2001) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Databáze: OpenAIRE