Limited Proteolysis and Its Influence on Thermal Stability of the Photosynthetic Reaction Center from Chloroflexus aurantiacus

Autor:	Nellie B. Levina, Michail A. Kutuzov, N.G. Abdulaev, Alexander S. Zolotarev
Rok vydání:	1990
Předmět:	Photosynthetic reaction centre Proteases medicine.diagnostic_test biology Chemistry Proteolysis Thermophile Chloroflexus aurantiacus Primary charge separation biology.organism_classification Photochemistry Chloroflexus medicine Thermal stability
Zdroj:	Molecular Biology of Membrane-Bound Complexes in Phototrophic Bacteria ISBN: 9781475708950
DOI:	10.1007/978-1-4757-0893-6_35
Popis:	Limited proteolysis with proteases of different specificity has been used to obtain a number of preparations of the reaction center (RC) from the thermophilic green bacterium Chloroflexus aurantiacus. The absorption spectrum of the native RC are preserved in these preparations, even after the shortening of the N-termini of both RC subunits to different extent. Considerable part of the hydrophilic N-terminus of the L-subunit has been shown to be unaccessible to the proteases. AlaL3-ArgL25 region contribute to the thermal stability of the Chloroflexus RC.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::84594fd4dc3223d93a629416b336af03 https://doi.org/10.1007/978-1-4757-0893-6_35 Zobrazit plný text záznamu