2,4,6-trinitrobenzenesulfonic acid-induced colitis in Rattus norgevicus: a categorization proposal
| Autor: | Marisa Esteves-Monteiro, Mariana Ferreira-Duarte, Salomé Gonçalves-Monteiro, Sara Capas-Peneda, Fernando Magro, Tiago Rodrigues-Pinto, Margarida Duarte-Araújo, Manuela Morato, Patrícia Dias-Pereira, Daniela Menezes-Pinto |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Food intake Body weight Inflammatory bowel disease Gastroenterology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Fluid intake 0302 clinical medicine 2 4 6-Trinitrobenzenesulfonic acid Internal medicine medicine Colitis Severe colitis General Veterinary business.industry General Medicine medicine.disease digestive system diseases 030104 developmental biology chemistry Categorization Animal Science and Zoology business 030217 neurology & neurosurgery |
| Zdroj: | Experimental Animals. 70:245-256 |
| ISSN: | 1881-7122 1341-1357 |
| Popis: | Reproducibility in animal research is crucial for its reliance and translational relevance. The 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced model of inflammatory bowel disease (IBD) is widely used but inconsistently and incompletely characterized throughout the literature. This hinders comparisons between studies and influences the low rate of translation of effective preclinical molecules. The purpose of this study was to categorize TNBS-induced colitis, based on macroscopic and microscopic scoring systems, and to identify basic routine parameters that could anticipate those categories. We retrospectively analysed male Wistar Rattus norvegicus (n=28 for the control group and n=87 for the TNBS group) and categorized TNBS-induced colitis in three phenotypes: Mild, Moderate and Severe colitis, as for human IBD. Also, we showed that the time course of food intake and fecal excretion (but not body weight, fluid intake or welfare scores) could foresee those categories. So, routine evaluation of food intake and fecal excretion may guide researchers in planning their experiments, selecting the animals with the severity of colitis that better matches their aims, or applying early humane endpoints to animals that will not be used in the experiments. In conclusion, categorizing TNBS-induced colitis enhances the reproducibility of data gathered with this experimental model and strengths its translational relevance. |
| Databáze: | OpenAIRE |
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