Increased gene expression of Toll-like receptor 4 on peripheral blood mononuclear cells in patients with psoriasis

Autor: Pilar Navarro, Jaime Sancho, J. Orgaz-Molina, L. Castellote, Esther Zumaquero, Sonia García-Rodríguez, S. Arias-Santiago, J.C. Ruiz, Rubén Perandrés-López, Mercedes Zubiaur, Agustín Buendía-Eisman
Rok vydání: 2011
Předmět:
Zdroj: Journal of the European Academy of Dermatology and Venereology. 27:242-250
ISSN: 0926-9959
DOI: 10.1111/j.1468-3083.2011.04372.x
Popis: Background A role for the innate immune system in driving the autoimmune T cell cascade in psoriasis has been proposed. Toll-like receptors-(TLR)-2 and -4 play a role in inflammation, atherosclerosis, and their specific role in psoriasis remains unclear. Objective To evaluate TLR2 and TLR4 gene expression levels in peripheral blood mononuclear cells from psoriatic patients. Methods Changes in TLR2 ⁄ 4 gene expressions were evaluated using quantitative real-time reverse transcription polymerase chain reaction in peripheral blood mononuclear cells, from twenty-one patients with severe psoriasis, and analysed whether there was any correlation with cytokine plasma levels (T-helper 0-, T-helper 1-, T-helper 2- or regulatory T cells-type), or Calprotectin and with S100A8 and S100A9 gene expression levels. Eleven non-psoriatic healthy controls were analysed. Results A clear increase in TLR4 gene expression was observed (3.84 ± 0.93, n = 21) together with a moderate increase in TLR2 expression (1.522 ± 0.31, n = 21). Both TLR4 and TLR2 gene expressions were significantly augmented in psoriatic patients compared with controls (all P Conclusion Differential TLR4 ⁄ 2 gene expressions on psoriatic peripheral blood mononuclear cells and correlations with regulatory and ⁄ or proinflammatory cytokines and ⁄ or damage-associated molecular pattern molecule S100A9 emphasize innate immune response role in psoriasis.
Databáze: OpenAIRE
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